Alterations in Airway Inflammation and Lung Function During Corticosteroid Therapy for Atopic Asthma: Statistics

The intraindividual ratios of change after 2 weeks of therapy (measure at 2 weeks divided by measure at baseline) and 8 weeks of therapy (measure at 8 weeks divided by measure at baseline) were calculated for each parameter. Spearman correlation coefficients between ratios of change at both time intervals were calculated using SAS software (SAS Institute; Cary, NC). The significance of changes after 2 weeks and after 8 weeks of inhaled corticosteroid therapy was estimated by analysis of variance (ANOVA).
Results
The data presented in this article were obtained during a biopsy-controlled study of the effects of inhaled FP on airway inflammation. Twenty-seven subjects were recruited. Four subjects were withdrawn: two were withdrawn due to noncompliance with medication (placebo [n = 1], FP [n = 1]), and two were withdrawn due to pregnancy (placebo [n = 1], FP [n = 1]). Eighteen atopic asthmatic subjects (placebo [n = 9], FP [n = 9]) completed the protocol. www.canadian-familypharmacy.com comments Individual measures of lung function and airway inflammation for the nine atopic subjects who received inhaled FP and completed the protocol are presented in Figures 1-3. Other details of dropouts, adverse events, and changes in lung function and airway inflammation in the placebo-treated subjects have been published previously.
Correlations Between Changes in Lymphocytes, Macrophages, and Eosinophils
After a 2-week interval, changes in T cells, CD45Ro lymphocytes, and CD68+ cells were all significantly correlated with each other (Table 2). Changes in eosinophils (EG1 or EG2) were not associated with changes in T cells, CD45Ro lymphocytes, or CD68+ cells (Table 2).
After an 8-week interval, changes in T cells, CD45Ro, and CD68 cells were all significantly correlated with each other (Table 3). Changes in eosinophils (EG1 or EG2) were not associated with changes in T cells, CD45Ro lymphocytes, or CD68 + cells (Table 3). Changes in EG1 were associated with changes in EG2 after 8 weeks of therapy (Table 3). There was no association between changes in EG1 and EG2 after 2 weeks of therapy.
Fig1
Figure 1. Measures of T cells (top, A), CD45Ro primed T lymphocytes (center, B), and CD68+ macrophages (bottom, C) in bronchial biopsy samples in subjects with atopic asthma (n = 9) at baseline, and after 2 weeks and 8 weeks therapy with high-dose inhaled FP, 2,000 ^g/d. *p < 0.01, ANOVA; **p < 0.001, ANOVA.
Fig2
Figure 2. Measures of EG1 eosinophils (top, A) and EG2 + eosinophils (bottom, B) in bronchial biopsy samples in subjects with atopic asthma (n = 9) at baseline, and after 2 weeks and 8 weeks of therapy with high-dose inhaled FP, 2,000 ^g/d. **p < 0.001, ANOVA.
Fig3
Figure 3. Measures of postbronchodilator FEV1 (top, A), bron-chodilator responsiveness (center, B), and PC20 (bottom, C) in subjects with atopic asthma (n = 9) at baseline, and after 2 weeks and 8 weeks of therapy with high-dose inhaled FP, 2,000 ^g/d. **p < 0.001, ANOVA.

Table 2—Spearman Rank Correlation Coefficients Between Changes in Bronchial Inflammation After 2 Weeks of Inhaled Corticosteroid Therapy (n = 9)

Variables EG2 EG1 CD68 CD45Ro
T cells 0.12 (0.75) 0.0 (1.0) 0.8 (< 0.01) 0.93 (< 0.001)
CD45Ro – 0.08 (0.83) 0.2 (0.6) 0.75 (0.02)
CD68 0.22 (0.6) 0.4 (0.29)
EG1 0.21 (0.6)

Table 3—Spearman Rank Correlation Coefficients Between Changes in Bronchial Inflammation After 8 Weeks of Inhaled Corticosteroid Therapy (n = 9)

Variables EG2 EG1 CD68 CD45Ro
T cells 0.2 (0.6) 0.2 (0.6) 0.83 (0.005) 0.83 (0.005)
CD45Ro – 0.36 (0.33) – 0.47 (0.2) 0.73 (0.02)
CD68 – 0.13 (0.7) – 0.2 (0.7)
EG1 0.84 (0.01)
anosiahuman.com