Archive for the ‘Cystic Fibrosis’ Category

Analysis of Sequential Aliquots of Hypertonic Saline Solution-Induced Sputum: Summary

Microbiology results suggested SI may be a useful tool in the multicentered research setting. Aliquots of induced sputum appear to provide a representative sample as quantitative bacterial cultures only varied by approximately a half-log count over time. In standard induced-sputum sample processing, di-thiothreitol has to be added to the sputum sample to homogenize it. However, […]

Analysis of Sequential Aliquots of Hypertonic Saline Solution-Induced Sputum: Asthmatic subjects

SI was relatively well tolerated by all subjects, However, two subjects had a > 15% fall in FEVj with this procedure even when premedicated with albuterol, However, the adverse effects associated with the sedation and the procedure of bronchoscopy with BAL on pulmonary function and oxygenation in patients with CF is not well reported but […]

Analysis of Sequential Aliquots of Hypertonic Saline Solution-Induced Sputum: Inflammatory Mediators

There was a mean half-log decrease in both nonmucoid P aeruginosa and S aureus with time (p = 0.05 and p < 0.01, respectively). The mean changes in nonmucoid P aeruginosa density and S aureus were 0.0.54 log10 cfu/mL (95% CI, — 1.60 to 0.52) and — 0.55 log10 cfu/mL (95% CI, — 1.42 to […]

Analysis of Sequential Aliquots of Hypertonic Saline Solution-Induced Sputum: Urea

Urea was detected in all subjects and in all samples. The mean urea concentration was 4 ± 1 mg/dL, 3 ± 1 mg/dL, 3 ± 1 mg/dL, 4 ± 1 mg/dL, and 3 ± 1 mg/dL at the five time points. The mean change from the 0- to 4-min sample to the 16- to 20-min […]

Analysis of Sequential Aliquots of Hypertonic Saline Solution-Induced Sputum: Sample Size Calculations

The null hypothesis for these tests was that sampling times, taken together, had no effect on the outcome of interest. A test for linear time trend was also performed for each outcome measure by fitting a regression in which time was modeled as a continuous variable. Finally, the mean change calculated as the 20-min value […]

Analysis of Sequential Aliquots of Hypertonic Saline Solution-Induced Sputum: Quantitative Culture

Samples were run in triplicate and compared to a standard curve. In order to ensure that dithiothreitol did not degrade IL-8, we added 100 ng and 500 ng IL-8 for 90 min to each sample from three patients (ie, total of 15 samples) and generated standard curves. Dithiothreitol did not degrade IL-8. Urea concentration was […]

Analysis of Sequential Aliquots of Hypertonic Saline Solution-Induced Sputum: SI

Using a modification of the protocol described by Fahy et al, modified by the Cystic Fibrosis Foundation Therapeutics Development Network, subjects inhaled nebulized sterile hypertonic saline (3%) solution for 20 min from an Ultra-Neb 99 ultrasonic nebulizer (DeVilbiss; Somerset, PA). This nebulizer generates particles of a mean mass median diameter of 4.5 μm and has […]

Analysis of Sequential Aliquots of Hypertonic Saline Solution-Induced Sputum: Materials and Methods

We have previously shown that SI may provide a more representative airway sample than BAL in patients with CF. When SI is compared with BAL and expectorated sputum (ES), SI had a lower percentage of squamous cells than ES, all three techniques had a similar nonsquamous cell differential count, quantitative microbiology correlated best between ES […]

Analysis of Sequential Aliquots of Hypertonic Saline Solution-Induced Sputum

Hypertonic saline has recently been used as a form of airway clearance in patients with cystic fibrosis (CF),-2 and as a means to obtain a lower airway sample for clinical use from patients who are unable to expectorate spontaneously. However, in high concentration (10%), hypertonic saline solution has been shown to cause bronchospasm in some […]

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