Archive for the ‘Dimethanesulfonate’ Category

Gestational Exposure to Ethane Dimethanesulfonate: DISCUSSION(4)

Therefore, it is possible that EDS may exert direct effects on both LCs and the seminiferous tubule. Alternatively, the effects on the LCs may represent indirect action resulting from direct seminiferous tubule toxicity. Finally, the effects on the seminiferous tubules may represent an indirect action resulting from direct LC toxicity. Regardless, it is now clear […]

Gestational Exposure to Ethane Dimethanesulfonate: DISCUSSION(3)

At adulthood, EDS-exposed mice still manifest incomplete spermatogenesis and numerous tubule cross sections still lacked germ cells. Similar disruption of spermatogenesis was previously observed in adult EDS-exposed mice 3 wk after five daily treatments (25 mg EDS/100 g BW) in the absence of apparent effects on the interstitium or LCs. It is well accepted that […]

Gestational Exposure to Ethane Dimethanesulfonate: DISCUSSION(2)

It was possible that the elevated ex vivo T production in the adult merely resulted from an increase in number of LCs rather than an increase in LH stimulation per LC. Although it appeared that there was indeed an increase in LC numbers in the EDS-exposed males, it was necessary to perform stereologic analysis to […]

Gestational Exposure to Ethane Dimethanesulfonate: DISCUSSION(1)

Although indices of normal sexual development such as AGD and time to preputial separation were altered, these changes most likely were due, not to compromised fetal T production, but rather to the EDS-induced decrease in body weight of the male offspring. Fetal whole-body and serum T values in normal male mice were consistent with previous […]

Gestational Exposure to Ethane Dimethanesulfonate: RESULTS(3)

By contrast, although some seminiferous tubules in EDS-exposed mice appeared normal, other tubule cross-sections continued to have incomplete spermatogenesis with Sertoli cell only tubules. Morphometric analysis revealed a decrease in the seminiferous tubule area, 57 516 ± 535 vs. 52 835 ± 1203.56 |xm2, an increase in the corresponding interstitial area, 7728 ± 535 vs. 12 […]

Gestational Exposure to Ethane Dimethanesulfonate: RESULTS(2)

The steroidogenic capacity of the LCs was not significantly affected in either unstimulated or hCG-stimulated prepubertal testis parenchyma from control and EDS exposed male offspring on PND 24, although there were trends toward increases in T production under both unstimulated and hCG-stimulated conditions (Fig. 3). Likewise, there was a trend toward increased unstimulated T production […]

Gestational Exposure to Ethane Dimethanesulfonate: RESULTS(1)

Administration of 160 mg/kg EDS during GD 11-17 did not affect overall maternal weight gain; however, it did decrease neonatal body weight on PND 1 by 15%, 1.72 vs. 1.47 g. This decrease was statistically significant and persisted in both the prepubertal and adult offspring. Both AGD and the onset of preputial separation, sensitive indicators […]

Gestational Exposure to Ethane Dimethanesulfonate: MATERIALS AND METHODS(12)

Thus, roundness could be assumed. Briefly, the area and volume of an individual LC were approximated by taking the average of the longest and shortest diameter, dividing it in half to yield the average radius, and then applying the calculations for area (A = п X radius2) and volume (V = 4/3[ п X radius3]). […]

Gestational Exposure to Ethane Dimethanesulfonate: MATERIALS AND METHODS(11)

Slides were incubated at room temperature for 30 min in rabbit anti-Cytochrome P450scc (#RDI-P450sccabr, Research Diagnostics, Inc.; Flanders, NJ), diluted 1: 200 in PBS containing 1.0% BSA, and then washed two times in PBS. Following a 1-h incubation at room temperature with biotinylated antirabbit IgG (H+L) (#BA-10000; Vector Laboratories) diluted 1:100 in PBS, sections were […]

Gestational Exposure to Ethane Dimethanesulfonate: MATERIALS AND METHODS(10)

For immunocytochemical/stereological studies, testes were removed from 70% ethanol, rinsed two times in 95% ethanol for 15 min, three times in 100% ethanol for 20 min, and two times in 100% xylene for 30 min. Tissue was incubated in a 1:1 xylene:paraffin (Paraplast Plus; Oxford Labwares, St. Louis, MO) bath at 60°C, two times for […]

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