Serial sections of approximately 800-A thickness were cut of several contiguous alveoli. Each specimen was serially sectioned to depths varying from 31 to 83 p.. At intervals of every tenth to 20th section (0.08 to 1.6 p.m), the complete circumference of each alveolar profile was photographed by successive overlapping exposures (approximately 20 to 30 per […]
Archive for the ‘Effects of Injury’ Category - Part 2
The fixed lungs were washed with fresh 0.1-M phosphate buffer. Biopsy specimens from representative areas of the treated lungs were taken from areas marked by the India ink to insure that the area had been exposed to either papain or physiologic saline. Specimens were prepared for light microscopy and for scanning and transmission electron microscopy. […]
Prcparation of Lung Specimens Six lung specimens were obtained for ultrastructural study as follows: two were control lungs from normal dogs (untreated controls); one was from the animal into which 24 ml of physiologic saline solution containing 1 mg of India ink had been instilled into the right lung (saline control); the fourth lung was […]
The manner of exposing the canine lung to papain and the preparation of the lung specimens has been described by Parra et al’ Briefly, conditioned animals were used after a 30-day period of observation. They were debarked and treated for ectoparasites and endoparasites and immunized against rabies, distemper, leptospirosis, hepatitis and parvovirus. They were anesthetized […]
In addition, increased amounts of oxygen radicals and peroxidase which can inactivate alpha-1 proteinase inhibitor or alpha-2 macroglobulin, another proteinase inhibitor, have been found in alveolar macrophages. Finally, cigarette smoke has been reported to stimulate cathepsin-B activity in the alveolar macrophages of rats. Cathepsin-B is one of the major enzymes with elastolytic activity produced by […]
The acute and subacute changes in lung remodeling resulting from proteolytic agents which produce severe pulmonary emphysema are not well understood. A canine model was developed in which severe emphysema is produced in only one lung, allowing survival of the animal so that such changes can be studied.