Archive for the ‘Gastric mucosal hydrophobicity’ Category

Human gastric mucosal hydrophobicity and Helicobacter pylori infection: Discussion (Part 5)

A possible explanation for this discordance in the results is the geographical variation in the diet, because, as shown by Lichtenberger et al , the surface active phospholipids are important determinants for gastric mucosal hydrophobicity. The present study highlights the fact that the hypothesis that H pylori causes peptic ulcers by reducing the mucosal layer […]

Human gastric mucosal hydrophobicity and Helicobacter pylori infection: Discussion (Part 4)

In a model of a mouse-adapted H pylori strain (SS1) used to infect C57BL/6 and gld mice, the contact angle readings were obtained by the ADSA-D technique. However, Lichtenberger et al reported reductions in the hydrophobicity of gastric mucosa following Helicobacter felis infection of C57BL/6 mice, and proposed that the reduction in surface hydrophobicity is […]

Human gastric mucosal hydrophobicity and Helicobacter pylori infection: Discussion (Part 3)

There are problems associated with using biopsy specimens . First, the biopsy size limits the volume of water droplets that can be formed on the mucus surface, thereby reducing the accuracy of the surface property determinations. Second, the biopsy surfaces are rough and heterogeneous, leading to the production of sessile drops. Finally, the trauma involved […]

Human gastric mucosal hydrophobicity and Helicobacter pylori infection: Discussion (Part 2)

Asante et al observed that H pylori-infected patients with duodenal ulcer had lower gastric hydrophobicity than infected patients with gastritis alone, but it was not clear whether it was H pylori infection or the ulceration that initiated the reduction in hydrophobicity. Second, they did not age and sex match the patients and controls in their […]

Human gastric mucosal hydrophobicity and Helicobacter pylori infection: Discussion (Part 1)

The measurement of contact angles in the determination of surface hydrophobicity, which is a biophysical property of tissue surfaces, yields values that closely agrees with those obtained by methods that do not rely on contact angles. Previous studies in humans have reported decreased gastric hydrophobicity with H pylori infection. In the present study, there was […]

Human gastric mucosal hydrophobicity and Helicobacter pylori infection: Results (Part 3)

To determine the impact of the severity of gastritis on the surface hydrophobicity measurements, the mean contact angle was compared in groups of patients with normal histology and those with different grades of gastritis based on antral histology. There was no significant difference (P=0.599, one-way ANOVA) between the groups. In addition, when both the factors […]

Human gastric mucosal hydrophobicity and Helicobacter pylori infection: Results (Part 2)

Considering the sex of the patient, there was no significant difference (P=0.277) in the mean contact angle between males (62.4±2.2°, n=42) and females (66.1±2.4°, n=57). To evaluate the effect of age on gastric mucosal hydrophobicity, patients were divided into three age groups that reflected the age distribution in the entire study group: group I, 40 […]

Human gastric mucosal hydrophobicity and Helicobacter pylori infection: Results (Part 1)

Of the 99 patients studied, 69 were H pylori-negative and 30 were H pylori-positive; of these 30, 10 were cagA+, 18 were cagA- and two were undetermined. Nine patients had atrophic gastritis, of which six were infected with cagA+. The mean contact angle for the 99 patients was 64.5±0.8°, with values ranging from 21.0° to […]

Human gastric mucosal hydrophobicity and Helicobacter pylori infection: Contact angle measurement

The fresh biopsy on the glass slide was placed on the goniometer stage and a 0.5 ^l drop of 0.9% saline deposited from a 0.2 mL microsyringe (Gilmont instruments, GS-1100, USA). Droplets were illuminated by a sodium lamp and imaged with a zoom lens (Navitar Inc, USA) coupled via an extension tube to a charge-coupled […]

Human gastric mucosal hydrophobicity and Helicobacter pylori infection: Methods

H pylori infection was assessed by histology, culture and PCR. Patients were considered to be H pylori-positive if two or more of the above tests were positive. Identification of the H pylori cagA+ strain was carried out by PCR using two pairs of primers: cagA (primer 1: 5′-CTC AAA TCC CCC TTA CCA AAC TC-3′; […]

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