Archive for the ‘Heart’ Category - Part 3

Hyperosmolar Solutions Stimulate Mucus Secretion in the Ferret Trachea: Discussion

We demonstrated that hyperosmolar solutions stimulated both mucin and ly-sozyme secretion, suggesting that either there is co-regulation of secretion or that both mucous and serous cells respond in the same way to the hyperosmolar agents. This is supported by the observation that the rate of mucin secretion was approximately two times that of lysozyme secretion […]

Hyperosmolar Solutions Stimulate Mucus Secretion in the Ferret Trachea: Evaluation of Secretory Pathways

There was 47% increase in lysozyme secretion with exposure to 3.69 g/dL of saline solution and a 54% increase with exposure to 10.69 g/dL of saline solution (each n = 5, p < 0.05) compared with exposure to KHS (Fig 2). The amounts of lysozyme secretion in tracheae exposed to KHS, 3.69 g/dL of saline […]

Hyperosmolar Solutions Stimulate Mucus Secretion in the Ferret Trachea: Statistical Analysis

Morphologic Evaluation of the Tracheal Epithelium Tracheal segments (n = 4 each) were taken before and after immersion in 3.69 g/dL of saline solution (1,192 mOsm), 10.69 g/dL of saline solution (3,612 mOsm), or in KHS (288 mOsm). Tissue segments were immersed in 10% formalin and processed for light (wide-field) microscopy. Paraffin-embedded tissue was cut […]

Hyperosmolar Solutions Stimulate Mucus Secretion in the Ferret Trachea: Lysozyme Secretion by Spectrophotometry

In brief, a 96-well microtiter plate was coated with 60 |j,L of DBA (6 |j,g/mL in phosphate-buffered saline solution [PBS]) and incubated at room temperature for 2 h. After rinsing with high-salt PBS (PBS containing 0.5 mol/L NaCl and 0.1% Tween 20), the plate was exposed to sample buffer and incubated at room temperature for […]

Hyperosmolar Solutions Stimulate Mucus Secretion in the Ferret Trachea: Mucin Secretion by Enzyme-Linked Lectin Assay

We randomized the administration of agents to different segments in each experiment. There is anatomic variation in the amount of ferret tracheal mucin secretion, with significantly greater secretion on the proximal (cephalad) trachea than near the carina. We eliminated anatomic variation in secretion as a potential confounding variable by ensuring that each study group had […]

Hyperosmolar Solutions Stimulate Mucus Secretion in the Ferret Trachea: Mucin and Lysozyme Secretion

Adult ferrets (1.7 to 2.3 kg, random sex) obtained from Marshall Farms (North Rose, NY) were killed by intraperitoneal injection of pentobarbital sodium (150 mg/kg body weight), and the trachea from the larynx to the carina was immediately removed. Each trachea was divided into four or eight roughly equal segments from the upper trachea to […]

Hyperosmolar Solutions Stimulate Mucus Secretion in the Ferret Trachea

Sputum induction using hypertonic saline solution inhalation has been used to obtain sputum specimens for diagnosing airway infection and for collecting secretions for research. More than 20 years ago, it was shown that when patients with chronic bronchitis inhaled a 7% (7 g/dL, 2,396 mOsm/L) saline aerosol, whole-lung mucociliary clearance (MCC) increased by 100% in […]

Postural Response of Low-Frequency Component of Heart Rate Variability: Limitations

Because this is a retrospective cohort study of prognosis, it is subject to all of the limitations of such studies. Particularly, the prognosis of CAD could depend on therapeutic strategies selected by physicians. We observed, however, that there were no group differences in the frequency of coronary interventions performed during the follow-up (Table 3) and […]

Postural Response of Low-Frequency Component of Heart Rate Variability: LF

However, there is also much evidence suggesting vagal involvement in LF. The LF decreases with strenuous exercise and vagal blockade with atro-pine, and it increases with sleep at night. The LF is decreased in patients with severe congestive heart failure, a state known as the loss of vagal-cardiac restraint. Additionally, the LF may result from […]

Postural Response of Low-Frequency Component of Heart Rate Variability: Possible Mechanisms for Postural LF Rise

The HF was assessed with the same method and the same conditions as were used in a previous study in which we observed the strong correlation between the HF and the level of cardiac vagal tone as assessed by pharmacologic autonomic blockades. In the present study, however, we also observed that a decreased HF had […]

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