Archive for the ‘Ovary’ Category - Part 2

Medroxyprogesterone Acetate: RESULTS(6)

Comparisons between ewes with normal and inadequate CL after the final ovulation of the study period. After the ovulation following MAP treatment of treated ewes, eight ewes formed as many CL as ovulated follicles (ewes with normal CL), but six ewes had fewer CL than ovulations (three treated and three control ewes; ewes with inadequate […]

Medroxyprogesterone Acetate: RESULTS(5)

There was a significant difference between the treated and control ewes in terms of progesterone concentrations after ovulation following MAP treatment in treated ewes. The treated ewes exceeded control ewes in mean serum concentrations of progesterone on Day 4 (1.49 ± 0.33 vs. 1.03 ± 0.22 ng/ml) and Day 5 (2.41 ± 0.29 vs. 1.32 […]

Medroxyprogesterone Acetate: RESULTS(4)

Progesterone concentrations and CL/luteal structures. Mean daily serum concentrations of progesterone for the ewes given PGF2c, and MAP and for control ewes are shown in Figure 2. All treated ewes responded to PGF2a as evidenced by an abrupt decline in circulating concentrations of progesterone and luteal regression observed with ultrasonography. Circulating concentrations of luteal progesterone declined […]

Medroxyprogesterone Acetate: RESULTS(3)

At the time of sponge removal in treated ewes, the last follicular wave before ovulation contained ovulatory follicles in all ewes studied. Of all follicles reaching >5 mm in diameter in this wave. 85% (11/13) and 73% (11/15) ovulated in the treatment and control groups, respectively (P > 0.05). The penultimate wave contained ovulatory follicles […]

Medroxyprogesterone Acetate: RESULTS(2)

The last follicular wave detected before the PGF2c,/MAP treatment of treated ewes emerged, on the average. 1.9 ± 0.2 days before the day of PGF2c, injection (range. 1 to 3 days), in both treatment and control groups (P > 0.05). During the 6-day period in which treated ewes had MAP sponges in place, follicles from […]

Medroxyprogesterone Acetate: RESULTS(1)

Experiment 1 Follicular dynamics and ovulations. Individual follicle profiles (follicles that grew from 3 to >5 mm in diameter) are shown for two different animals for each group in order to illustrate the pattern of follicular wave development and the number and time of emergence of ovulatory follicles during the study period (Fig. 1). Within […]

Medroxyprogesterone Acetate: MATERIALS AND METHODS(7)

Therefore, a follicular wave was defined as one or more antral follicles that grew from the pool of 2 to >3-mm follicles to >5 mm in diameter (emergent follicles) within 48 h. Analyses for each hormone were performed in single assays. The intraassay CVs for mean LH concentrations of 0.49 or 0.98 ng/ml were 7.6% […]

Medroxyprogesterone Acetate: MATERIALS AND METHODS(6)

Experiment 2 The first experiment was repeated to confirm the ovulations that occurred during sponging and the effects of MAP treatment on ovulation rate after sponge withdrawal. In addition, we examined the gonadotropin dependency of the ovulations that occurred during MAP treatment. Ewes were treated later in the breeding season (December-January) than they were in […]

Medroxyprogesterone Acetate: MATERIALS AND METHODS(5)

Daily serum concentrations of FSH, estradiol, and progesterone for all ewes were aligned with the day of PGF2„ treatment for the treated ewes (Day 0), and analyzed for the period from Day —1 to Day 7. Daily concentrations of the hormones were also aligned with the day of ovulation after the MAP treatment period (Day […]

Medroxyprogesterone Acetate: MATERIALS AND METHODS(4)

Hormone analyses. Serum samples were analyzed by RIA for concentrations of LH, FSH, estradiol, and progesterone. Earlier studies in our laboratory (unpublished) showed that the antiserum to progesterone did not cross-react with MAP which permitted the measurement of endogenous progesterone in MAP-treated ewes. Concentrations of LH and FSH are given in terms of NIAMDD-oLH-24 and […]

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