Diethylstilbestrol Versus Estradiol: MATERIALS AND METHODS(3)

METHODS(3)

The harvested tracts were ultimately divided into cervix, uterine horns, and oviduct/ovarian (when present) regions and then embedded in paraffin. For the present study, 4- to 5-^m midfrontal sections from the cervical region of the tracts were processed for light microscopy using standard hematox-ylin-and-eosin staining. At least four sections cut from each of the cervices harvested from all of the animals per time point and treatment group were analyzed. Digital images were captured with a Kodak MDS 290 system (Eastman Kodak Co., Rochester, NY). The photomicrographs shown are representative of the histomorphological conditions in the triplicate specimens from the three treatment groups at every observation point.

Using photomicrographs, cervical diameters were determined by comparing manual measurements of tissue section dimensions (at the uterine/endocervical junction) to those of an objective micrometer.

Statistics

For the data regarding animal weight, cervical diameter, and normalized cervical diameter, an SAS statistical package was used to calculate the mean, the SEM, and the significance levels of differences among the three neonatal treatment groups at all the time points. Factorial AN-OVA was followed by the Tukey honestly significant difference test for multiple comparisons, and means were considered to be significantly different at P < 0.05.

Category: Estradiol

Tags: cervix, developmental biology, estradiol, female reproductive tract, toxicology