Diethylstilbestrol Versus Estradiol: RESULTS(2)
They provide visual confirmation that both neonatal treatment regimens enhanced cervical growth as early as Day 3, but then (Day 5 to Day 21) that response was more dramatic in the neonatally DES-exposed animals than in the neonatally E2-exposed animals. Another anatomical feature that can be appreciated in Figures 2-4 (and in subsequent figures that contain low-magnification micrographs) is that the hamster has a true duplex uterus with two separate cervical canals, each with their own external os. Still another finding evident even at low magnification was that early cytodiffer-entiation of the luminal epithelium in the ectocervical region was altered to a different extent by neonatal DES treatment than by neonatal E2 treatment.
For instance, as early as Day 5, precocious cornification of the stratified squamous epithelium was obvious in the DES-exposed cervix (Fig. 4). That phenomenon is illustrated at higher magnification in Figure 5 and is contrasted to what occurs in the columnar epithelium that lines the lumen in the uterine region of the hamster reproductive tract. In the ectocervical region of the 5-day-old tracts (Fig. 5, left), stratification of the luminal epithelium was 1) incipient in control animals; 2) multilayered, but noncornified, in neonatally E2-treated animals; and 3) extensively cornified in neonatally DES-treated animals (indicated by an asterisk in Fig. 5).
FIG. 5. Effects of neonatal DES versus E2 exposure on the luminal epithelium of the uterine and ectocervical regions of reproductive tracts from 5-day-old hamsters. Using the same representative sections shown for Day 5 in Figures 2 (control [CON]), 3 (E2), and 4 (DES), separate high-magnification micrographs were taken from the cranial (cr) or uterine-adjacent region and from the caudal (ca) or vaginal-adjacent region. Each micrograph shows the cellular organization of a length of luminal epithelium (E) and a portion of its underlying mesenchymal or stromal tissue (S). The asterisk marks a region where the stratified squamous epithelium was corni-fied (flattened, keratinized cells to the right of the asterisk and desquamated sheets of cells to the left).