Diethylstilbestrol Versus Estradiol: RESULTS(2)


They provide visual confirmation that both neonatal treatment regimens enhanced cervical growth as early as Day 3, but then (Day 5 to Day 21) that response was more dramatic in the neonatally DES-exposed animals than in the neonatally E2-exposed animals. Another anatomical feature that can be appreciated in Figures 2-4 (and in subsequent figures that contain low-magnification micrographs) is that the hamster has a true duplex uterus with two separate cervical canals, each with their own external os. Still another finding evident even at low magnification was that early cytodiffer-entiation of the luminal epithelium in the ectocervical region was altered to a different extent by neonatal DES treatment than by neonatal E2 treatment.

For instance, as early as Day 5, precocious cornification of the stratified squamous epithelium was obvious in the DES-exposed cervix (Fig. 4). That phenomenon is illustrated at higher magnification in Figure 5 and is contrasted to what occurs in the columnar epithelium that lines the lumen in the uterine region of the hamster reproductive tract. In the ectocervical region of the 5-day-old tracts (Fig. 5, left), stratification of the luminal epithelium was 1) incipient in control animals; 2) multilayered, but noncornified, in neonatally E2-treated animals; and 3) extensively cornified in neonatally DES-treated animals (indicated by an asterisk in Fig. 5).
Fig5Diethylstilbestrol Versus-5
FIG. 5. Effects of neonatal DES versus E2 exposure on the luminal epithelium of the uterine and ectocervical regions of reproductive tracts from 5-day-old hamsters. Using the same representative sections shown for Day 5 in Figures 2 (control [CON]), 3 (E2), and 4 (DES), separate high-magnification micrographs were taken from the cranial (cr) or uterine-adjacent region and from the caudal (ca) or vaginal-adjacent region. Each micrograph shows the cellular organization of a length of luminal epithelium (E) and a portion of its underlying mesenchymal or stromal tissue (S). The asterisk marks a region where the stratified squamous epithelium was corni-fied (flattened, keratinized cells to the right of the asterisk and desquamated sheets of cells to the left).

Category: Estradiol

Tags: cervix, developmental biology, estradiol, female reproductive tract, toxicology