Diethylstilbestrol Versus Estradiol: RESULTS(3)

In the uterine-adjacent region of the same sections, histology of the luminal epithelium was distinct from that observed in the ectocervical regions but mimicked what we had found previously in sections taken from the midregion of the uterus in 5-day-old hamsters. Specifically, in the lower uterine region adjacent to the cervix (Fig. 5, right), the columnar epithelium was low cuboidal in control and neonatally E2-treated animals but taller and pseudostratified in neonatally DES-treated animals.

Adult, Intact Animals

Effects of neonatal DES versus E2 treatment on body weight, absolute size (diameter) of the cervix, and size of the cervix normalized to body weight in 1- to 5-mo-old adult animals are shown in Figure 6. Body weight tended to be reduced by both neonatal treatment regimens, but the difference was statistically significant only for the DES-exposed versus the control group at the 3-mo time point. Consequently, body weight differences cannot account for the following treatment-induced differences in cervical size. At the earliest time point, cervical size in both absolute (Fig. 6B) and normalized terms (Fig. 6C) was enhanced in the neonatally DES-exposed group, but that effect was not statistically significant. Thereafter, cervical size was enhanced in both neonatal treatment groups. The enhancement in absolute cervical size was statistically significant at the 2- to 4-mo time points in the neonatally DES-exposed group and at the 3-mo time point in the neonatally E2-exposed group. The same was true for the enhancement of normalized cervical size, except that it was also statistically significant at the 5-mo time point in the neonatally DES-exposed group. my canadian neighbor pharmacy

Fig6Diethylstilbestrol Versus-6
FIG. 6. Effects of neonatal DES versus E2 treatment on body weight, absolute size of the cervix, and normalized size of the cervix in adult, intact hamsters. Following injection on the day of birth (Day 0) with vehicle alone (control [C]) or 100 |xg of either E2 (E) or DES (D), female animals at the indicated days of age were weighed (A), and their reproductive tracts were fixed, separated into various regions, and processed for standard paraffin embedding, sectioning, and hematoxylin and eosin staining. Cervical diameters were measured at the uterine/endocervix junction of mid-frontal tissue sections (see Fig. 2) and were expressed both on an absolute basis (B) and after being normalized to the animal’s body weight (C). Values represent the mean ± SEM (n = 3; errors bars not shown where variability was so small as to be masked by the data point), and means that are significantly different (P < 0.05) from each other at each time point are indicated by different lowercase letters.

Category: Estradiol

Tags: cervix, developmental biology, estradiol, female reproductive tract, toxicology