The differential effect of neonatal DES vs. E2 treatment on cervical size was modest and somewhat variable (Fig. 6, A and B), but the differential effect of the two treatments on histomorphology of the adult cervix was striking and very consistent from the 2-mo time point onward. For instance, the low-magnification micrographs in Figure 7 confirm the rank order of cervical size shown for the three groups of animals at the 2-mo time point shown in Figure 6 (DES > E2 > control). However, inspection of the same sections at higher magnification revealed that luminal epithelial histology in both the ectocervix (Fig. 8, left) and uterine-adjacent regions (Fig. 8, right) was quite different between the two neonatal treatment groups. buy cheap allegra
In the ecto-cervical region, the stratified squamous epithelium was multilayered, but noncornified, in both the control and neonatally E2-treated animals, but it was extensively cornified in the neonatally DES-treated animals (indicated by an asterisk in Fig. 8). In the uterine-adjacent region of the same sections, epithelial histology again was distinct from that observed in the ectocervical regions and again mimicked what we had found previously in sections taken from the midregion of adult uteri.
FIG. 7. Cervical histology in adult, intact hamsters. Shown are low-magnification micrographs of midfrontal sections of the cervical regions positioned with their cranial (uterine-adjacent) aspect to the right and caudal (vaginal-adjacent) aspect to the left. The tissues are from 2-mo-old hamsters that had been injected on the day of birth with vehicle alone (control [CON]) or containing 100 |xg of either E2 or DES and are representative of the histology observed in the cervices from three separate animals per treatment group.
FIG. 8. Effects of neonatal DES versus E2 exposure on the luminal epithelium of the uterine and ectocervical regions of reproductive tracts from adult, intact hamsters. Using the same representative sections shown and described in Figure 7, separate high-magnification micrographs were taken from the cranial (cr) or uterine-adjacent region and from the caudal (ca) or vaginal-adjacent region. Each micrograph shows the cellular organization of a length of luminal epithelium (E) and a portion of its underlying mesenchymal or stromal tissue (S). The asterisk marks a region where the stratified squamous epithelium was cornified.