Diethylstilbestrol Versus Estradiol: RESULTS(6)


These changes in absolute size of the cervix induced by neonatal treatment were not statistically significant (Fig. 9B), but they did follow the same pattern as that noted above for body weight. Consequently, normalized cervical size (Fig. 9C) was not affected by either neonatal treatment regimen in the O+E2 hamsters. Figure 9 also shows that, between the two time points studied, body weight increased, but absolute size of the cervix did not change. Thus, the normalized size of the cervix decreased in all three groups.

Cervical histomorphology was another characteristic that did not change between 1 and 2 mo of age within any of the three groups of O+E2 animals. canadian health&care mall inc

Consequently, only examples from the 2-mo time point are shown. The low-mag-nification micrographs of the representative tissue sections in Figure 10 confirm that neither neonatal treatment regimen altered cervical diameter to a significant extent. However, inspection of the same sections at higher magnification revealed that the O+E2 condition induced cornification of the squamous epithelium in the ectocervical region of all three groups (Fig. 11, left). That result contrasts with what was observed in the uterine-adjacent regions of the same sections (Fig. 11, right) and was found previously in sections taken from the miduterine region of adult, O+E2 hamsters. Specifically, the luminal epithelium in the lower uterine region adjacent to the cervix was moderately tall and pseudostratified in both the control and the neonatally E2-treated group. In the neonatally DES-treated group, it was extremely tall and disorganized, riddled with cavities that often contained degenerating cells, and again, the cell’s basal aspects were difficult to distinguish from the underlying stromal tissue compartment. Together, these results indicate that neonatal E2 versus DES treatment also has differential disruptive effects on the hamster cervix, but the phenomenon does not completely mimic that observed in the hamster uterus.
Fig11Diethylstilbestrol Versus-11
FIG. 11. Effects of neonatal DES versus E2 exposure on the luminal epithelium of the uterine and ectocervical regions of reproductive tracts from ovariectomized and estrogen-replaced adult hamsters. Using the same representative sections shown and described in Figure 10, separate high-magnification micrographs were taken from the cranial (cr) or uterine-adjacent region and from the caudal (ca) or vaginal-adjacent region. Each micrograph shows the cellular organization of a length of luminal epithelium (E) and a portion of its underlying mesenchymal or stromal tissue (S). The asterisks mark regions where the stratified squamous epithelium was cornified.

Category: Estradiol

Tags: cervix, developmental biology, estradiol, female reproductive tract, toxicology