Gestational Exposure to Ethane Dimethanesulfonate: INTRODUCTION(3)

INTRODUCTION(3)

Additionally, adverse functional effects on cultured Sertoli cells, including inhibition of the synthesis of the glycoprotein, transferrin, have been demonstrated at relevant sublethal in vitro doses of EDS (300-500 ^M). Declining levels of this soluble iron transport protein can result in decreased numbers of germ cells and suppression of spermatogenesis.

The purpose of this study was to determine whether gestational exposure to EDS would adversely affect steroidogenesis in the developing mouse fetus and, if so, whether this results in persistent reproductive effects in the prepubertal and adult mouse. To our knowledge, this is the first study employing gestational exposure of EDS in any species. This study was designed to examine the effects of gestational EDS exposure on T biosynthesis throughout reproductive development, the attainment of puberty, and subsequent reproductive performance in the adult mouse. Here we provide the first demonstration that fetal mouse LCs are sensitive to EDS, as shown by decreases in the fetal T peak alterations persisting into adulthood including incomplete spermatogenesis, altered LC structure and function, and reduced fertility.

Category: Dimethanesulfonate

Tags: Leydig cells, testis, testosterone, toxicology