Gestational Exposure to Ethane Dimethanesulfonate: MATERIALS AND METHODS(2)


Treatment Administration

Timed pregnant mice were randomly assigned to one of two treatment groups: vehicle (dimethyl sulfoxide [DMSO]: deionized water; 3:7, v/v) or EDS (gift from Sterling Research Group, Rensselaer, NY) (160 mg/kg body weight [BW] per day, in a 30% DMSO solution, i.p.) from GD 11 through GD 17; GD 0 = day of postcoital plug. A preliminary study in which pregnant dams were given 160 mg/kg EDS daily from GD 11-17 revealed no overt maternal toxicity, but male offspring had significantly reduced fetal T and persistent decreases in body weight, testis weight, and fertility. Therefore, the current study focused on this same dosage of EDS. The volume administered was equivalent to 0.2 ml per 25 g of maternal BW.

Handling and Offspring

For all end points, the litter became the experimental unit; therefore, litter mean data were analyzed and reported here. For endogenous whole-body T determination, both male and female fetuses and neonates were recovered via cesarean section on consecutive days from GD 15 to PND 4. For treatment-related effects on whole body T, time pregnant CD-1 dams were dosed from GD 11 to GD 17 with 160 mg/kg EDS.