Human gastric mucosal hydrophobicity and Helicobacter pylori infection: Contact angle measurement

The fresh biopsy on the glass slide was placed on the goniometer stage and a 0.5 ^l drop of 0.9% saline deposited from a 0.2 mL microsyringe (Gilmont instruments, GS-1100, USA). Droplets were illuminated by a sodium lamp and imaged with a zoom lens (Navitar Inc, USA) coupled via an extension tube to a charge-coupled device camera (Hamamatsu C3077, Hamamatsu Photonics Ltd, United Kingdom). The picture of the drop on the biopsy surface was then captured by the camera and transferred to the computer, where the sides of the drop were magnified and the angle readings taken at a high magnification.

For each biopsy, six contact angle readings were taken by applying three different saline drops (two readings/drop, one from each side). In addition, to assess repeatability, the contact angle readings for 22 patients were repeated (six additional readings for each biopsy, total 12 readings). The contact angle measurements representing the hydrophobicity of the gastric mucus layer were performed without prior knowledge of the patients’ H pylori status or histological findings. The contact angle was determined by measuring the angle between the solid-liquid line and the liquid-air line.

Results were analysed using SPSS software version 10.1 (SPSS Inc, USA) and expressed as mean ± SEM. Statistical significance was tested using ANOVA and Student’s t test (paired and unpaired). The level of statistical significance was taken at P<0.05. Pearson correlation coefficient was used in the determination of the associations between parameters. Find best deals an online pharmacy can offer and get a chance to start your treatment soon. You can now purchase antibiotic levaquin at the best pharmacy that will be sure to live up to your expectations every single time.

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