We randomized the administration of agents to different segments in each experiment. There is anatomic variation in the amount of ferret tracheal mucin secretion, with significantly greater secretion on the proximal (cephalad) trachea than near the carina. We eliminated anatomic variation in secretion as a potential confounding variable by ensuring that each study group had an equal number of segments from each portion of the trachea. Each tracheal segment was exposed to only one test agent.
The concentrations of saline solution used were based on published data demonstrating that the hypertonic saline solution increases mucociliary clearance at a concentration of 3 to 12%. The concentration of 14 g/dL of mannitol was chosen because it has an osmolarity similar to that of 3.69 g/dL of saline solution (1,040 mOsm vs 1,192 mOsm, respectively). The osmolarity of all solution was measured by freezing-point depression in the Clinical Laboratory Improvement Amend-ments-certified clinical laboratory of our hospital. Measurements were performed in triplicate and agreed within 0.5%. These results were also consistent with the calculated osmo-larity of saline solutions. These studies were approved by the Animal Care and Use Committee of Wake Forest University. my canadian pharmacy
Submucosal glands in the ferret trachea have both serous and mucous cells, and ferret tracheal mucins have high blood group titers, reflecting an abundance of galactose-N-acetyl-a1-3 (fu-cose-a1-2) galactose-R. These blood group antigens can be detected by the lectin Dolichos biflorus agglutinin (DBA). DBA immunohistochemistry staining shows specific binding to goblet cells and submucosal glands in the ferret trachea.
A sandwich enzyme-linked lectin assay was used to measure mucin secretion as previously described.