Infection of ileum mesothelium: Discussion (Part 1)

High power examination of the submucosal ulcer bed delineated rare spindle shaped cells with a ground glass nuclear appearance, circumscribed by a rim of dense chromatin. These cells also had irregular nuclear membranes and were occasionally multinucleated. Similar cytopathic atypia was in the vicinity of the serosa beneath the ulcer. No viral atypia was detected in endothelial cells or mucosal epithelium.

Cells with viral cytopathic effect in the ulcer bed had a HSV type I, HSV type II, vimentin and a focally actin-positive IHC phenotype. Such cells were negative for CMV, CD1a, CD68, CD45, CD20, CD31, S-100 and PGP9.5. Serosal cells with viral inclusions were HSV type I-, HSV type II-and calretinin-positive. These cells were also negative for those antigens that ulcer bed cells failed to react with.

ISH and PCR detected HSV DNA with a melting point characteristic of HSV type II (Figure 1). No CMV, adenovirus or VZV genetic material were detected. Your turn to find out more about the possibility to get and pay tons less.

HSV infection of adulthood is predominantly a disease of the immunocompromised. In the bone marrow transplant population, 70% of seropositive patients will reactivate, usually within the first month. These patients commonly have oral/genital mucositis with or without esophagitis. The rare patient develops HSV dissemination, primarily manifesting as encephalitis, pneumonia or hepatitis.

Polymerase chain reaction

Figure 1) Polymerase chain reaction identifies herpes simplex virus (HSV) DNA in the bowel (two separate paraffin blocks, A3 and A4) and real-time polymerase chain reaction delineates its melting point to be that of HSV type II

anosiahuman.com