Therefore, a follicular wave was defined as one or more antral follicles that grew from the pool of 2 to >3-mm follicles to >5 mm in diameter (emergent follicles) within 48 h.
Analyses for each hormone were performed in single assays. The intraassay CVs for mean LH concentrations of 0.49 or 0.98 ng/ml were 7.6% or 5.9%, respectively. For FSH reference sera with mean concentrations of 0.42 or 2.32 ng/ml, the intraassay CVs were 5.6% or 1.6%, respectively. Intraassay CVs for reference sera with mean progesterone concentrations of 0.45 or 1.22 ng/ml were 14.4% or 4.6%, respectively. Serum concentrations of LH and FSH in blood samples taken every 4 h were analyzed for the period from 1 day before to 1 day after MAP treatment in all ewes, and also for the period from 48 h before to 12 h after ovulations detected during the period of treatment with MAP sponges (breakthrough ovulations), in five of eight ewes studied. buy nexium online
The third experiment was undertaken to confirm and document the ovulations that occurred during sponging and to remove the ovaries for histology. Ewes were treated in September and October, as described for the first two experiments. Ovarian ultrasonography was performed every 12 h, starting 1 day before PGF2a injections and insertion of MAP sponges (8 days after ovulation), using an Aloka SSD-900 echo camera connected to a 7.5-MHz transducer. When ovulations were detected with ultrasonography, the ewes were transferred indoors and deprived of food for 12-18 h before surgery the following day. General anesthesia was induced with sodium pentobarbital (Somnotol, MTC Pharmaceutical, Cambridge, ON, Canada; 65 mg/ml; 20-30 ml/ewe, i.v.) and maintained with 2.5% halothane/oxygen (Halocarbon Laboratories, River Edge, NJ) administered by an endotracheal tube at an average flow rate of 0.75 L/min. Ovaries were exposed, photographed, and then dissected, and small tissue blocks containing ruptured follicles were fixed in Bouins solution. Paraffin wax sections were prepared and stained with hematoxylin and eosin. The histology of the ruptured follicles/CH was studied via light microscopy.