Current studies using postmortem brain tissue from postmenopausal women may be confirmed and extended in the NHP model. For example, hypertrophied neuronal tissue from the infundibular nucleus of postmenopausal women express estrogen receptor, neurokinin B, and substance P mRNAs. The GnRH gene expression increased in a separate subpopulation of neurons within the medial basal hypothalamus. Additional studies using postmortem brain tissue from postmenopausal women documented a decrease in the numbers of neurons expressing pro-opiome-lanocortin (POMC) gene transcripts. Because of the diverse role of POMC in various physiologic processes, this loss may considerably affect the physiology of postmenopausal women. my canadian pharmacy online
However, many inherent limitations exist in the use of human autopsy specimens. For example, because of the effects of stress on the reproductive axis, subject selection should be limited to patients experiencing sudden death and without a history of significant chronic illness. Collection of these materials is a slow process, and the acquisition of autopsy specimens from subjects with well-documented hormone replacement therapy is exceedingly difficult. More importantly, the interpretation of data comparing pre- and postmenopausal women is complicated by the presence of two major variables: age and ovarian status.