Serum Concentration of Soluble lnterleukin-2 Receptor as a Sensitive Parameter of Disease Activity in Sarcoidosis (4)
Assay for Serum Angiotensin Converting Enzyme Activity
The level of serum ACE activity was determined by the modified spectrophotometric assay of Cushman and Cheung as described previously. The values of less than 40 U/ml were considered normal.
Bronchoalveolar lavage fluid (BALF) was obtained within one week after serum samples were collected from nine patients with active untreated sarcoidosis. A flexible fiberoptic bronchoscope was wedged in a segmental bronchus of the right middle lobe and three 50-ml aliquots of 0.9 percent sterile saline solution were injected. Total cell number was calculated and a cytocentrifiige preparation was stained with Wright-Giemsa staining. A differential cell count was obtained by counting 500 cells, and surface markers of lymphocytes were examined by using FITC-conjugated anti Ieu4/PE-conjugated anti leu 12 solution (CD3VCD194 Simultest T and B Cell Test, Becton, Dickinson & Co, Oxnard, CA) and FITC-conjugated anti leu3a/PE-conjugated anti leu2a solution (CD4+/ CD8+ Simultest T Helper/Suppresser Test, Becton, Dickinson & Co, Oxnard, CA). Fluorescence intensity of each sample was measured by using fluorescence-activated cell sorter (FACStar, Becton, Dickinson & Co, Oxnard, CA). buy flovent inhaler
The statistical significance of differences was determined by using Student s t test. Comparison between two parameters was made by using Spearmans regression analysis.